Category Archives: Electrophysiology

Visualizing recording pipettes with quantum dots

Here’s a quick description/’review’ of the method for making pipettes fluorescent by quantum dot coating described in Andrásfalvy et al 2014. We used the protocol for some in-vivo 2p guided sharps recordings a while ago and really liked how cheap, simple … Continue reading

Posted in Electrophysiology | Leave a comment

Cheap dental drill

Common dental drills are useful/required to have in any systems lab. In addition to the usual applications, these can be used to cut holes in cover slides (diamond abrasive burrs), to cut small openings in drive implant bodies, smooth out dental cement, … Continue reading

Posted in Calcium imaging, Electrophysiology | Comments Off on Cheap dental drill

High quality time series plots

What is the best, or at least a good way to plot time-series data on a screen? When dealing with time series data in electrophysiology, a good deal of time is spent looking at plots in order to judge data … Continue reading

Posted in Data analysis, Electrophysiology, Open Ephys | Comments Off on High quality time series plots

Opinion piece on open-source electrophysiology

Josh and I (of Open Ephys), Greg Hale (of Arte) and Jon Newman (of Neurorighter, Cyclops & Puggle) just published an opinion piece on the role of open-source approaches and interfaces for large-scale electrophysiology. Its a bit of a review of … Continue reading

Posted in Electrophysiology, Open Ephys | Comments Off on Opinion piece on open-source electrophysiology

Recording simultaneous units in cortex with the flexDrive

We’ve been using the flexDrive (wiki) for over a year now, recording almost 100 sessions in 5 mice. I’m just now starting to analyze neural ensemble statistics that require simultaneously recorded neurons. Here’s the real-world distribution of how many simultaneous neurons … Continue reading

Posted in Electrophysiology, Technical things | Comments Off on Recording simultaneous units in cortex with the flexDrive

Digital fine wire tether

For recordings in freely behaving mice, it is important to minimize the weight and torque applied by the cables. This is especially important for experiments that require natural behavior and becomes a real issue for channel counts over 32 where even light … Continue reading

Posted in Electrophysiology, Open Ephys | Comments Off on Digital fine wire tether

Localizing recording sites: Reconstructing explanted electrode arrays

While drive implants with adjustable electrodes bring many advantages, the use of movable electrodes adds one element of uncertainty to the data analysis: the precise depth of each electrode is not known with absolute certainty during recording. By identifying landmarks along the … Continue reading

Posted in Data analysis, Electrophysiology | Comments Off on Localizing recording sites: Reconstructing explanted electrode arrays

The flexDrive implant

Our drive implant design, the flexDrive just got published at Frontiers Systems Neuroscience: Voigts J, Siegle JH, Pritchett DL and Moore CI (2013). The flexDrive: An ultra-light implant for optical control and highly parallel chronic recording of neuronal ensembles in … Continue reading

Posted in Electrophysiology, Open Ephys | Comments Off on The flexDrive implant

New headstages for open-ephys

Josh and I just assembled the first prototypes for the new and improved 32 channel headstages for the open-ephys system, an open source system for extra-cellular electrophysiology. These are smaller than the previous ones even though they now feature a standard omnetics … Continue reading

Posted in Electrophysiology, Open Ephys | Comments Off on New headstages for open-ephys